Regulatory
Part:BBa_K3179003:Design
Designed by: Jiaxian Xu Group: iGEM19_SYSU-CHINA (2019-10-13)
miR592-target
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part, four repeating sequences complementary to miRNA592 on an mRNA, is a 4X miRNA target designed to target miRNA592. When miRNA592 reaches a certain concentration in the cell, it would combine with miR592-target and suppress the translation of mRNA. According to our design, miR592-target is designed to control the translation of mRNA with response parts. We integrate miR592-target with L7Ae so that L7Ae cannot be translated with miRNA592 existing in the cell.
Source
We get this sequence from NCBI, and the sequence was synthesized from a biological company.
References
1 Bartel D P. MicroRNAs: target recognition and regulatory functions[J]. cell, 2009, 136(2): 215-233.